Bioassay LOD Fitter

A web application for analysing concentration-response data, performing advanced sigmoidal curve fitting, and rigorously calculating the Limit of Detection (LOD).

Bioassay LOD Fitter (v0.8.0) is a web application built to analyse concentration-response data, perform advanced sigmoidal curve fitting, and calculate the Limit of Detection (LOD) for biological and chemical assays using validated statistical frameworks (Currie 1968, Holstein et al. 2015).

Key Features

  • Multi-Curve Plot Overlay & Leaderboard: Overlay multiple curves (e.g. Wild-Type vs Mutants, Buffer A vs B) on a shared broken logarithmic axis with color-coordinated trendlines, interactive hover-focus, and a sortable Multi-Curve Sensitivity Leaderboard displaying fold-change shifts.
  • Direct Spreadsheet Clipboard Paste: Copy rows and columns directly from Microsoft Excel or Google Sheets (Ctrl+V / Cmd+V) into the sidebar for instant table parsing.
  • Serial Dilution Auto-Generator: Quick-configure standard dilution series with automated concentration calculation (start concentration, dilution factor, and replicate count).
  • Interactive Chart Layer Toggles: Custom toggle pills for 95% CI, LC/LD lines, LOD Zone, and Grid to inspect raw curves without visual clutter.
  • Unified Single-View Analytics: View the prominent LOD Hero Card, fitted parameter micro-grid, interactive AICc comparison table, and collapsible statistical noise limits ($L_C, L_D$, blank SD, pooled SD) in a cohesive single view without tab-hopping.
  • Completely client-side processing: Fast, secure local calculations without uploading sensitive biological or chemical data to external servers.
  • Interactive Multi-Model Comparison: Renders a live statistical table showing R² and AICc metrics side-by-side for Linear, Langmuir, 4PL, and 5PL models, with dynamic best-fit gold-star selectors and one-click Auto (AICc) optimization.
  • Assay Quality Checks Widget: Automated diagnostic checks that flag high replicate variance (CV%), poor fits, or non-monotonic trends.
  • Continuous Hover Telemetry Tooltips: Smooth fade-in tooltips showing predicted values, 95% Confidence Interval bounds, and raw replicates at any coordinate, accompanied by dashed crosshair cursor guides.
  • Bidirectional Table-to-Chart Highlights: Hovering over individual data points highlights the exact replicate in the calibration data table, while row hover illuminates corresponding replicate circles on the chart.
  • Publication-Ready Export: Download high-resolution 300 DPI PNG plots, lossless vector SVG, and comprehensive CSV / Markdown statistical reports.